Hi CHARMm users,
I build a system which contains RNA, protein and a membrane with Cholesterol so i need to read in the parameter files for all of the four components (NA, PROT, LIPID, CHOL).
I checked each structure by its own and made sure each works.
But I failed at the point where I want to put the system together. I couldn't manage to read all the required rtf/prm files.
I tried different combinations and orders but no success.
See an example below:
! Read topology and parameter files
open read card unit 10 name para/top_all27_prot_na.rtf
read rtf card unit 10
open read card unit 20 name para/par_all27_prot_na.prm
read para card unit 20
open read card unit 10 name para/top_all27_lipid.rtf
read rtf card append unit 10
open read card unit 20 name para/par_all27_lipid.prm
read para card append unit 20
Before I start to post all possible errors I collected I would like to ask if this is the proper way of reading multiple rft/prm files. If not, please let me know how to read all the needed parameters (NA, PROT, LIPID, CHOLESTEROL)