Here are the .ret and .prm files of the engineered deprotonated LYR(ChR2) which can be recognized by ChARMM-GUI. And they refer to this file(toppar_all36_prot_retinol.str) and this paper(Zhu S, Brown MF, Feller SE. Retinal conformation governs pKa of protonated Schiff base in rhodopsin activation. J Am Chem Soc. 2013 Jun 26;135(25):9391-8. doi: 10.1021/ja4002986. Epub 2013 Jun 11. PMID: 23701524; PMCID: PMC5176254.).
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I'm so sorry! The ".ret" means ".rtf" file. That was a writing blunder. Achieve this by above-mentioned references. It's different from the LYR in toppar_all36_prot_retinol.str which makes every LYR protonated. However, LYR needs to be deprotonated at times. It's developed by understanding the charmm ff and referring to literature. And it can be used in CHARMM-GUI with engineered residues option(PDB reader or Membrane Builder). You can get a deprotonated LYR when you upload "LYR.zip".